|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13444||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
Vector typeMammalian Expression, Cre/Lox
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2800
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), PGK polyA site, and 3x SV40 polyA site (tpA). The PGKneotpA is flanked by loxP sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PGKneotpAlox2 was a gift from Philippe Soriano (Addgene plasmid # 13444 ; http://n2t.net/addgene:13444 ; RRID:Addgene_13444)
For your References section:Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P. Nat Genet. 1999 Jan . 21(1):70-1. 10.1038/5007 PubMed 9916792