STOP-eGFP-ROSA26TV
(Plasmid #11739)

• Depositing Lab: Klaus Rajewsky
• Publication: Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

Backbone

• Vector backbone: pBSII KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Cre/Lox ; /STOP
• Selectable markers: Neomycin (select with G418) ; Diptheriatoxin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STOP-eGFP-ROSA26TV
• Species: DNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
• Insert Size (bp): 11500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: T7/M13
• 3′ sequencing primer: T7/M13

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Based on pRosa26-1 from Soriano P, Fred Hutchinson, Cancer Research Institute
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Targeting vector for cDNA knock-in into the Rosa26 locus.

See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912