pBTG (pBigT-IRES-GFP; DM#268)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15037||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7562
Vector typeCre/Lox ; for making transgenic constructs
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
/ Fusion Protein
- IRES-EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypBigT from Frank Costantini.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.
Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBTG (pBigT-IRES-GFP; DM#268) was a gift from Douglas Melton (Addgene plasmid # 15037 ; http://n2t.net/addgene:15037 ; RRID:Addgene_15037)
For your References section:Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333