Purpose(Empty Backbone) Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21270||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||21270-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Backbone manufacturerCostantini Lab
- Backbone size (bp) 6117
Vector typeMouse Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.
Information for Cloning Grade DNA (Catalog # 21270-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBigT was a gift from Frank Costantini (Addgene plasmid # 21270 ; http://n2t.net/addgene:21270 ; RRID:Addgene_21270)
For your References section:Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. BMC Dev Biol. 2001 . 1():4. 10.1186/1471-213X-1-4 PubMed 11299042