Purpose(Empty Backbone) Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21270||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCostantini Lab
- Backbone size (bp) 6117
Vector typeMouse Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBigT was a gift from Frank Costantini (Addgene plasmid # 21270 ; http://n2t.net/addgene:21270 ; RRID:Addgene_21270)
For your References section:Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. BMC Dev Biol. 2001 . 1():4. 10.1186/1471-213X-1-4 PubMed 11299042