pET15b CnA CnB
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11787||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5700
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCnA CnB
Alt namePpp3ca (calcineurin A subunit alpha)
Alt namePpp3r1 (calcineurin B, type I)
SpeciesH. sapiens (human)
Entrez GenePPP3R1 (a.k.a. CALNB1, CNB, CNB1)
Entrez GenePPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B)
- Promoter T7
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET15b CnA CnB was a gift from Anjana Rao (Addgene plasmid # 11787 ; http://n2t.net/addgene:11787 ; RRID:Addgene_11787)
For your References section:Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center. Mondragon A, Griffith EC, Sun L, Xiong F, Armstrong C, Liu JO. Biochemistry. 1997 Apr 22. 36(16):4934-42. 10.1021/bi9631935 PubMed 9125515