pC-KIT2
(Plasmid
#118984)
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PurposeMutational dissection of the G rich region of the c-kit promoter
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Depositing Lab
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Depositing OrganizationKarolinska Institutet
Located in Sweden -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 118984-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 118984-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepsiCHEK2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5589
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-kit proto-oncogene {promoter}
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SpeciesH. sapiens (human)
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Insert Size (bp)1215
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MutationmutK2-SP-K1
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GenBank IDS67773.1
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Entrez GeneKIT (a.k.a. C-Kit, CD117, MASTC, PBT, SCFR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 118984-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 118984-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC-KIT2 was a gift from Bjorn Hogberg (Addgene plasmid # 118984 ; http://n2t.net/addgene:118984 ; RRID:Addgene_118984) -
For your References section:
Interplay of Three G-Quadruplex Units in the KIT Promoter. Ducani C, Bernardinelli G, Hogberg B, Keppler BK, Terenzi A. J Am Chem Soc. 2019 Jul 3;141(26):10205-10213. doi: 10.1021/jacs.8b12753. Epub 2019 Jun 20. 10.1021/jacs.8b12753 PubMed 31244182
Map uploaded by the depositor.
