LV-CIG
(Plasmid #12107)

• Depositing Lab: Inder Verma
• Publication: Pfeifer et al Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5.

Backbone

• Vector backbone: n/a
• Backbone size w/o insert (bp): 6670
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: nlsCre
• Alt name: nls Cre
• Alt name: LV-Cre
• Alt name: cre
• Species: Enterobacteriophage P1
• Insert Size (bp): 2476
• Mutation: IRES GFP C-terminal to the nlsCre. Bicistronic expression of nlsCre and GFP.
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry