PurposeThis plamid was used for improving the production of DMAPP (IPP) in E.coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121149||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCDF- Duet-1
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Streptomycin, 50 μg/mL
Copy numberLow Copy
Gene/Insert nameMVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMVA was a gift from Minjuan Xu (Addgene plasmid # 121149 ; http://n2t.net/addgene:121149 ; RRID:Addgene_121149)
For your References section:Combinatory Biosynthesis of Prenylated 4-Hydroxybenzoate Derivatives by Overexpression of the Substrate-Promiscuous Prenyltransferase XimB in Engineered E. coli. He BB, Bu XL, Zhou T, Li SM, Xu MJ, Xu J. ACS Synth Biol. 2018 Sep 21;7(9):2094-2104. doi: 10.1021/acssynbio.8b00070. Epub 2018 Aug 30. 10.1021/acssynbio.8b00070 PubMed 30103600