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pMVA2RBS035
(Plasmid #121051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121051 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 121051-D50 50 μg of DNA in Tris buffer $495
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DNA 121051-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBbA1k-RFP
  • Backbone manufacturer
    Lee, Taek Soon, et al. "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biologica

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mvaE
  • Species
    Enterococcus faecalis

Gene/Insert 2

  • Gene/Insert name
    mvaS
  • Species
    Enterococcus faecalis

Gene/Insert 3

  • Gene/Insert name
    mvaK1
  • Species
    Streptococcus pneumoniae

Gene/Insert 4

  • Gene/Insert name
    mvaK2
  • Species
    Streptococcus pneumoniae

Gene/Insert 5

  • Gene/Insert name
    mvaD
  • Species
    Streptococcus pneumoniae

Gene/Insert 6

  • Gene/Insert name
    idi
  • Species
    Escherichia coli

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMVA2RBS035 was a gift from Nigel Scrutton (Addgene plasmid # 121051 ; http://n2t.net/addgene:121051 ; RRID:Addgene_121051)

  • For your References section:

    Machine learning of designed translational control allows predictive pathway optimisation in Escherichia coli. Jervis AJ, Carbonell P, Vinaixa M, Dunstan MS, Hollywood KA, Robinson CJ, Rattray NJW, Yan C, Swainston N, Currin A, Sung R, Toogood HS, Taylor S, Faulon JL, Breitling R, Takano E, Scrutton NS. ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398. 10.1021/acssynbio.8b00398 PubMed 30563328
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