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pIREShyg-PA-GFP-alpha Tubulin
(Plasmid #12296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12296 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 12296-D50 50 μg of DNA in Tris buffer $495
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DNA 12296-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIREShyg2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha Tubulin
  • Alt name
    PA-GFP-Tubulin
  • Alt name
    k-alpha-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Tag / Fusion Protein
    • PA-GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PA-GFP-Tubulin came from the pPA-GFP-alpha Tubulin vector

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIREShyg-PA-GFP-alpha Tubulin was a gift from Patricia Wadsworth (Addgene plasmid # 12296 ; http://n2t.net/addgene:12296 ; RRID:Addgene_12296)

  • For your References section:

    Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells. Tulu US, Rusan NM, Wadsworth P. Curr Biol. 2003 Oct 28. 13(21):1894-9. 10.1016/j.cub.2003.10.002 PubMed 14588246
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