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H2B-GFP
(Plasmid #11680)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11680 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human histone H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    380
  • GenBank ID
    X00088
  • Entrez Gene
    HIST1H2BJ (a.k.a. H2B/r, H2BFR, H2BJ)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Two sequence differences from consensus sequence (D26G and V119I) were detected in Addgene sequencing. This clone is as originally published and isolated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680 ; http://n2t.net/addgene:11680 ; RRID:Addgene_11680)
  • For your References section:

    Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Kanda T, Sullivan KF, Wahl GM. Curr Biol. 1998 Mar 26. 8(7):377-85. 10.1016/S0960-9822(98)70156-3 PubMed 9545195