|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14757||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6122
- Total vector size (bp) 6953
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemembrane bound form of EGFP
Alt namegreen fluorescent protein
Insert Size (bp)831
- Promoter pCAG
/ Fusion Protein
- Palmitoylation signal (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bymGFP (Okada et al. Exp. Neurol. 156, 394-406 (1999)) was obtained from Dr. S. McConnell (Stanford Univ.)
Terms and Licenses
Articles Citing this Plasmid
mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.
The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-mGFP was a gift from Connie Cepko (Addgene plasmid # 14757 ; http://n2t.net/addgene:14757 ; RRID:Addgene_14757)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010