pCAG-mGFP
(Plasmid #14757)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 5. ():.

Backbone

• Vector backbone: pCAGEN
  (Search Vector Database)
• Backbone size w/o insert (bp): 6122
• Total vector size (bp): 6953
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: membrane bound form of EGFP
• Alt name: GFP
• Alt name: green fluorescent protein
• Insert Size (bp): 831
• Promoter: pCAG
• Tag / Fusion Protein:
  • Palmitoylation signal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: mGFP (Okada et al. Exp. Neurol. 156, 394-406 (1999)) was obtained from Dr. S. McConnell (Stanford Univ.)
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 32 References

Depositor Comments

mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.

The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.

Information for Cloning Grade DNA (Catalog # 14757-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $95 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here