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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 14757 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAGEN
- Backbone size w/o insert (bp) 6122
- Total vector size (bp) 6953
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemembrane bound form of EGFP
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Alt nameGFP
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Alt namegreen fluorescent protein
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Insert Size (bp)831
- Promoter pCAG
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Tag
/ Fusion Protein
- Palmitoylation signal (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bymGFP (Okada et al. Exp. Neurol. 156, 394-406 (1999)) was obtained from Dr. S. McConnell (Stanford Univ.)
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Terms and Licenses
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Articles Citing this Plasmid
Depositor Comments
mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.
The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-mGFP was a gift from Connie Cepko (Addgene plasmid # 14757 ; http://n2t.net/addgene:14757 ; RRID:Addgene_14757) -
For your References section:
Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010