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pCAG-mGFP
(Plasmid #14757)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14757 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 14757-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 6122
  • Total vector size (bp) 6953
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    membrane bound form of EGFP
  • Alt name
    GFP
  • Alt name
    green fluorescent protein
  • Insert Size (bp)
    831
  • Promoter pCAG
  • Tag / Fusion Protein
    • Palmitoylation signal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.

The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.

Information for Cloning Grade DNA (Catalog # 14757-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mGFP was a gift from Connie Cepko (Addgene plasmid # 14757 ; http://n2t.net/addgene:14757 ; RRID:Addgene_14757)
  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010