pCAG-GFP
(Plasmid #11150)

• Purpose: Mammalian expression vector for expression of GFP (CAG promoter)
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
• Citations: 214 Articles

Backbone

• Vector backbone: pCAGEN
• Backbone manufacturer: Available at Addgene (#11160)
• Backbone size w/o insert (bp): 4779

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP from Aequorea victoria
• Alt name: GFP
• Alt name: green fluorescent protein
• Insert Size (bp): 772

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EGFP was from pEGFP-N1 (Clontech).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this region of the plasmid.