pEF-GFP
(Plasmid #11154)

• Purpose: Mammalian expression vector for expression of GFP (EF1a promoter)
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
• Citations: 52 Articles

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 3086
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1 alpha promoter
• Alt name: EF1A1 promoter
• Alt name: eukaryotic translation elongation factor 1 alpha 1
• Alt name: EF1A1
• Species: H. sapiens (human)
• Insert Size (bp): 1188
• Entrez Gene: EEF1A1 (a.k.a. CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The EF1 alpha promoter (Kim et al., Gene 91, 217-273 (1990)) was obtained from Dr. S. Sugano (Univ. of Tokyo).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry