pCAGIG
(Plasmid #11159)

• Purpose: Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
• Citations: 95 Articles

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4817
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRES-EGFP
• Alt name: internal ribosomal entry site from Encephalomyocarditis virus
• Alt name: green fluorescent protein from Aequorea victoria
• Insert Size (bp): 1318

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: MscI (destroyed during cloning)
• 5′ sequencing primer: pCAG-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: IRES-GFP was from pMX-IRES-GFP (Nosaka et al., EMBO J. 18, 4754-4765 (1999)) obtained from Dr. T. Kitamura (Univ. of Tokyo).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PstI site at 3112 and the HindIII site at 3120 shown in the author's map and sequence are not present in this plasmid.