PurposeExpression of a membrane-targeted form of eGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61099||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4731
Modifications to backboneThis modified GFP is myristoylated and targeted to the plasma membrane (rafts). It was constructed from a parent Lck-GFPN1 construct. The parent Lck sequence was removed and replaced with a new Lck sequence containing two glycine residues at the C-term of Lck, as opposed to an isoleucine and a valine. The new Lck sequenced was prepared by PCR with BglII at the 5’ end and EcoRI at the 3’ end. The parent Lck was digested with BglII and EcoRI, removing the old Lck sequence, and insertng the new Lck using the same restriction sites.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40 poly A Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySee publication for cloning details.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lck-GFP was a gift from Steven Green (Addgene plasmid # 61099 ; http://n2t.net/addgene:61099 ; RRID:Addgene_61099)
For your References section:Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures. Benediktsson AM, Schachtele SJ, Green SH, Dailey ME. J Neurosci Methods. 2005 Jan 30;141(1):41-53. 10.1016/j.jneumeth.2004.05.013 PubMed 15585287