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mCherry-Mem
(Plasmid #55779)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55779 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Derived from pEYFP-Mem
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3942
  • Total vector size (bp) 4783
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-Mem
  • Species
    Discosoma sp.
  • Insert Size (bp)
    841
  • Promoter CMV
  • Tag / Fusion Protein
    • The N-terminal 20 residues of neuromodulin are fused to the N-terminus of mCherry.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer sV40pA-Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry was obtained from Roger Tsien, University of California, San Diego, CA. pEYFP-Mem was purchased from Clontech.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for mCherry:
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004;22(12):1567-72. PubMed PMID: 15558047.

The K196R mutation in mCherry has no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Mem was a gift from Catherine Berlot (Addgene plasmid # 55779 ; http://n2t.net/addgene:55779 ; RRID:Addgene_55779)
  • For your References section:

    Live cell analysis of G protein beta5 complex formation, function, and targeting. Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. 10.1124/mol.107.038075 PubMed 17596375