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Live cell analysis of G protein beta5 complex formation, function, and targeting.
Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
55707Cer(1-158)-beta-1 in pcDNAI/AmpAn amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.
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55708Cer(1-158)-beta-5 in pcDNAI/AmpAn amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
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55760Cer(1-158)-RGS7An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
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55761CFP(1-158)-RGS7tAn amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
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55762YFP(1-158)-gamma-2 in pcDNAI/AmpAn amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
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55763CFP(159-238)-beta-5 in pcDNAI/AmpA carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.
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55776G protein alpha-o-YFPThis G protein alpha-o construct contains internal insertions of YFP and the EE epitope
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55777CFP(159-238)-gamma-2 in pcDNAI/AmpA C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.
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55778Cer(159-238)-beta-5 in pcDNAI/AmpA carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.
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55779mCherry-MemThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.
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55780G protein alpha-o-R179C-EEThis is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
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55781G protein alpha-s-YFPThis G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
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55782G protein alpha-q-YFPThis G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
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60898Cerulean(159-238) in pcDNAI/AmpThis is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.
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