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G protein alpha-s-YFP
(Plasmid #55781)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55781 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA1/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7103
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-s-EE YFP
  • Alt name
    Gnas
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2303
  • Mutation
    Met was substituted for Gln69 in EYFP (Clontech). BamHI sites in the alpha-s cDNA were removed and alpha-s residues 73-84 were replaced with Gly-Ser adding a unique BamHI site.
  • Entrez Gene
    Gnas (a.k.a. ALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EYFP flanked by SGGGS on each side was inserted in place of alpha-s residues 73-84.
    • internal EE epitope (residues 189-194 in alpha-s sequence are EYMPTE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-s-YFP was a gift from Catherine Berlot (Addgene plasmid # 55781 ; http://n2t.net/addgene:55781 ; RRID:Addgene_55781)
  • For your References section:

    Live cell analysis of G protein beta5 complex formation, function, and targeting. Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. 10.1124/mol.107.038075 PubMed 17596375