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G protein alpha-o-YFP
(Plasmid #55776)


Item Catalog # Description Quantity Price (USD)
Plasmid 55776 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7639
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    G protein alpha-o internally tagged with EYFP and EE epitope
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    A BglII site was removed from the 5' untranslated region of alpha-oEE and then a unique BglII site was introduced in frame between Pro119 and Phe120 into which EYFP containing a substitution of Met for Gln69 and flanked on both ends by a SGGGGS linker was inserted as a BamHI cassette (destroying both the BglII and BamHI sites).
  • Entrez Gene
    Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EYFP containing a Q69M mutation and flanked on both ends by a SGGGGS linker was inserted between Pro119 and Phe120.
    • EE epitope was introduced internally with D167E and Q169M mutations.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pEYFP was purchased from Clontech.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-o-YFP was a gift from Catherine Berlot (Addgene plasmid # 55776 ; ; RRID:Addgene_55776)
  • For your References section:

    Live cell analysis of G protein beta5 complex formation, function, and targeting. Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. 10.1124/mol.107.038075 PubMed 17596375