Venus2-DeltaCT-STAT3
(Plasmid
#123179)
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PurposeExpresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversidade Nova de Lisboa, Instituto de Tecnologia Quimica e Biologica Antonio Xavier NOVA (ITQB NOVA)
Located in Portugal -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 123179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 123179-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 123179-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA 3.1
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 5440
- Total vector size (bp) 7447
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Modifications to backboneNone
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTAT3 tagged with Venus2 (aa 158-238)
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SpeciesH. sapiens (human)
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Insert Size (bp)2007
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MutationDeletion of the SH2 and TAD domains (C-terminus, aa 585-770)
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GenBank IDBC014482.1
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Entrez GeneSTAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
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Tag
/ Fusion Protein
- Venus2 (aa 158-238) for bimolecular fluorescence complementation assays (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 123179-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 123179-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Venus2-DeltaCT-STAT3 was a gift from Federico Herrera (Addgene plasmid # 123179 ; http://n2t.net/addgene:123179 ; RRID:Addgene_123179) -
For your References section:
Can asymmetric post-translational modifications regulate the behavior of STAT3 homodimers?. Letra-Vilela R, Cardoso B, Silva-Almeida C, Maia Rocha A, Murtinheira F, Branco-Santos J, Rodriguez C, Martin V, Santa-Marta M, Herrera F. FASEB Bioadv. 2020 Jan 27;2(2):116-125. doi: 10.1096/fba.2019-00049. eCollection 2020 Feb. 10.1096/fba.2019-00049 PubMed 32123861
