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pLdCN2
(Plasmid #125186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125186 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 125186-D50 50 μg of DNA in Tris buffer $495
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DNA 125186-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pLdCN
  • Vector type
    CRISPR ; Leishmania
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA and Cas9
  • Species
    Leishmania

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbs I (unknown if destroyed)
  • 3′ cloning site Bbs I (unknown if destroyed)
  • 5′ sequencing primer SP6 promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene sequence was derived from plasmid pX330 from Dr Feng Zhang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Leishmania CRISPR vector pLdCN2 was specially designed to facilitate cloning two gRNA guide coding sequences into the vector with a single-step digestion-ligation cloning protocol.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLdCN2 was a gift from Greg Matlashewski (Addgene plasmid # 125186 ; http://n2t.net/addgene:125186 ; RRID:Addgene_125186)

  • For your References section:

    Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania. Zhang WW, Lypaczewski P, Matlashewski G. Methods Mol Biol. 2020;2116:199-224. doi: 10.1007/978-1-0716-0294-2_14. 10.1007/978-1-0716-0294-2_14 PubMed 32221923
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