PurposeExpress gRNA and Cas9 in Leishmania with Neomycin resistance
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84290||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 9217
Vector typeCRISPR ; Leishmania
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namegRNA and Cas9
gRNA/shRNA sequencenew gRNA guide
- Promoter L. donovani ribosome RNA promoter
- Cloning method Restriction Enzyme
- 5′ cloning site Bbs I (not destroyed)
- 3′ cloning site Bbs I (not destroyed)
- 5′ sequencing primer SP6 Promoter (Common Sequencing Primers)
This Leishmania CRISPR vector co-express gRNA and Cas9 with neomycin resistance. It can also be used to express dual, triple or more gRNAs simultaneously with Cas9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLdCN was a gift from Greg Matlashewski (Addgene plasmid # 84290 ; http://n2t.net/addgene:84290 ; RRID:Addgene_84290)
For your References section:Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms. Zhang WW, Lypaczewski P, Matlashewski G. mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb. mSphere00340-16 [pii] PubMed 28124028