pLdCN
(Plasmid #84290)

• Purpose: Express gRNA and Cas9 in Leishmania with Neomycin resistance
• Depositing Lab: Greg Matlashewski
• Publication: Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb.

Backbone

• Vector backbone: pSP72
• Total vector size (bp): 9217
• Vector type: CRISPR ; Leishmania
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA and Cas9
• gRNA/shRNA sequence: new gRNA guide
• Species: Leishmania
• Promoter: L. donovani ribosome RNA promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bbs I (not destroyed)
• 3′ cloning site: Bbs I (not destroyed)
• 5′ sequencing primer: SP6 Promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pX330-U6-Chimeric_BB- CBh-hSpCas9 Obtained from Brodt Institute (through Addgene)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This Leishmania CRISPR vector co-express gRNA and Cas9 with neomycin resistance. It can also be used to express dual, triple or more gRNAs simultaneously with Cas9.