Addgene: Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.

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Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.
Zhang WW, Lypaczewski P, Matlashewski G
mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb. PubMed

Plasmids from Article

ID	Plasmid	Purpose
84290	pLdCN	Express gRNA and Cas9 in Leishmania with Neomycin resistance
84291	pLdCH	Express gRNA and Cas9 in Leishmania with Hygromycin resistance
84292	pSPneogRNA241510+MT	Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

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