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(Plasmid #125186)


Item Catalog # Description Quantity Price (USD)
Plasmid 125186 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    CRISPR ; Leishmania
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    gRNA and Cas9
  • Species

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbs I (unknown if destroyed)
  • 3′ cloning site Bbs I (unknown if destroyed)
  • 5′ sequencing primer SP6 promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene sequence was derived from plasmid pX330 from Dr Feng Zhang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Leishmania CRISPR vector pLdCN2 was specially designed to facilitate cloning two gRNA guide coding sequences into the vector with a single-step digestion-ligation cloning protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLdCN2 was a gift from Greg Matlashewski (Addgene plasmid # 125186 ; ; RRID:Addgene_125186)
  • For your References section:

    Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania. Zhang WW, Lypaczewski P, Matlashewski G. Methods Mol Biol. 2020;2116:199-224. doi: 10.1007/978-1-0716-0294-2_14. 10.1007/978-1-0716-0294-2_14 PubMed 32221923