Bov.hsc70.T77.A1-386.wt
(Plasmid #12534)

• Depositing Lab: David McKay
• Publication: Wilbanks et al J Biol Chem. 1994 Apr 29. 269(17):12893-8.

Backbone

• Vector backbone: pT7-7
• Backbone manufacturer: William Studier
• Backbone size w/o insert (bp): 2500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bovine 70 kDa heat shock protein ATPase fragment
• Alt name: ATPase fragment, bovine Hsc70
• Species: B. taurus (bovine)
• Insert Size (bp): 1158
• Mutation: deletion of residues 387-650 of bovine Hsc70 protein

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also see Structural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. II. Structure of the active site with ADP or ATP bound to wild type and mutant ATPase fragment. In J Biol Chem. 1994 Apr 29. 269(17):12899-907. (Pubmed: 8175707).