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pCAG-Flpe
(Plasmid #13787)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13787 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flpe
  • Alt name
    enhanced form of Flp recombinase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1332
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The coding sequence of Flpe was amplified by PCR using pCMVscript-FlpeERT2 (Hunter et al. Genesis 41, 99-109 (2005)) obtained from Dr. S. Dymecki (Harvard Medical School) as a template.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation. Myc-tag was added at the C-terminus of Flpe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Flpe was a gift from Connie Cepko (Addgene plasmid # 13787 ; http://n2t.net/addgene:13787 ; RRID:Addgene_13787)
  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010