PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14797||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4779
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCre-ERT2 fusion protein
Alt nameinducible Cre
Insert Size (bp)2004
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI and NotI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-CreERT2 was a gift from Connie Cepko (Addgene plasmid # 14797 ; http://n2t.net/addgene:14797 ; RRID:Addgene_14797)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010