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pCAG-ERT2CreERT2
(Plasmid #13777)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13777 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 4779
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERT2-Cre-ERT2 fusion protein
  • Alt name
    inducible Cre
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    2951

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

4-hydroxitamoxifen-responsible Cre.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI (SmaI) and NotI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-ERT2CreERT2 was a gift from Connie Cepko (Addgene plasmid # 13777)
  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010