PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13769||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6072
Vector typeMammalian Expression, Cre/Lox ; Flp/FRT
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt namered fluorescent protein
Insert Size (bp)728
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cre recombinase-dependent inducible expression vector. DsRed is expressed only in the presence of Cre.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCALNL-DsRed was a gift from Connie Cepko (Addgene plasmid # 13769 ; http://n2t.net/addgene:13769 ; RRID:Addgene_13769)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010