|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13786||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5555
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namemir30 cassette
SpeciesH. sapiens (human)
Insert Size (bp)205
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe mir30 cassette was derived from pSM2 (Open Biosystems).
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).
Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCALSL-mir30 was a gift from Connie Cepko (Addgene plasmid # 13786 ; http://n2t.net/addgene:13786 ; RRID:Addgene_13786)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010