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pCALSL-mir30
(Plasmid #13786)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13786 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 5555
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mir30 cassette
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    205

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mir30 cassette was derived from pSM2 (Open Biosystems).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).

Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCALSL-mir30 was a gift from Connie Cepko (Addgene plasmid # 13786 ; http://n2t.net/addgene:13786 ; RRID:Addgene_13786)
  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010