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MSCV CreERT2 puro
(Plasmid #22776)


Item Catalog # Description Quantity Price (USD)
Plasmid 22776 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMSCV puro
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor, which recognizes tamoxifen or its derivative 4-hydroxytamoxifen(4-OHT). This construct permits tamoxifen-dependent Cre activity.

Please note that Addgene's sequencing results identified a K183E mutation in the Cre protein sequence. This mutation was not intentional, but was present in the original plasmid and according to the depositing laboratory the plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV CreERT2 puro was a gift from Tyler Jacks (Addgene plasmid # 22776 ; ; RRID:Addgene_22776)
  • For your References section:

    Dicer1 functions as a haploinsufficient tumor suppressor. Kumar MS, Pester RE, Chen CY, Lane K, Chin C, Lu J, Kirsch DG, Golub TR, Jacks T. Genes Dev. 2009 Nov 10. ():. 10.1101/gad.1848209 PubMed 19903759