MSCV CreERT2 puro
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22776||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepMSCV puro
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor, which recognizes tamoxifen or its derivative 4-hydroxytamoxifen(4-OHT). This construct permits tamoxifen-dependent Cre activity.
Please note that Addgene's sequencing results identified a K183E mutation in the Cre protein sequence. This mutation was not intentional, but was present in the original plasmid and according to the depositing laboratory the plasmid functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV CreERT2 puro was a gift from Tyler Jacks (Addgene plasmid # 22776 ; http://n2t.net/addgene:22776 ; RRID:Addgene_22776)
For your References section:Dicer1 functions as a haploinsufficient tumor suppressor. Kumar MS, Pester RE, Chen CY, Lane K, Chin C, Lu J, Kirsch DG, Golub TR, Jacks T. Genes Dev. 2009 Nov 10. ():. 10.1101/gad.1848209 PubMed 19903759