PurposeMammalian expression of enhanced form of Flp recombinase fused to GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13788||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4779
Vector typeMammalian Expression ; Flp/FRT
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFlpe-GFP fusion protein
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2024
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe coding sequence of Flpe was amplified by PCR using pCMVscript-FlpeERT2 (Hunter et al. Genesis 41, 99-109 (2005)) obtained from Dr. S. Dymecki (Harvard Medical School) as a template. GFP was from pEGFP-N1 (Clontech).
Terms and Licenses
Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-Flpe:GFP was a gift from Connie Cepko (Addgene plasmid # 13788 ; http://n2t.net/addgene:13788 ; RRID:Addgene_13788)
For your References section:Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 5. ():. 10.1073/pnas.0610155104 PubMed 17209010