MSCV-human CSFI-R-L301S-IRES-GFP  
              
              
                (Plasmid
                
                #133744)
              
            
            
            
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            Purposeretroviral expression of CSF1-R mutant
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              Depositing Lab
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          Publication
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backboneMSCV LTR
 - Backbone size w/o insert (bp) 6415
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              Vector typeMammalian Expression, Retroviral
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                Selectable markersGFP
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)NEB Stable
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            Copy numberUnknown
 
Gene/Insert
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                Gene/Insert nameCSF1-R
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                  Alt namec-fms
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                    SpeciesH. sapiens (human)
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                  Insert Size (bp)3000
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                  MutationL301S
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                        Entrez GeneCSF1R (a.k.a. BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, GPSC, HDLS, HDLS1, M-CSF-R)
 - Promoter MSCV
 
Cloning Information
- Cloning method Restriction Enzyme
 - 5′ cloning site Not1 (unknown if destroyed)
 - 3′ cloning site Not1 (unknown if destroyed)
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-human CSFI-R-L301S-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 133744 ; http://n2t.net/addgene:133744 ; RRID:Addgene_133744) - 
                
For your References section:
Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ. Nature. 1987 Feb 5-11;325(6104):549-52. 10.1038/325549a0 PubMed 3027579