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PurposeVSV-G envelope expressing plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
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Vector backbonepMD2.G
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Vector typeMammalian Expression, Lentiviral ; Envelope
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVSV G
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Fwd (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid runs as a dimer (>11kb). While this may reduce DNA yield, the plasmid still functions as expected for viral packaging.
Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)
Map uploaded by the depositor.
