pMD2.G
(Plasmid #12259)

• Purpose: VSV-G envelope expressing plasmid
• Depositing Lab: Didier Trono
• Publication: Trono Lab Packaging and Envelope Plasmids (unpublished)
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Full (1) Partial (4)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	12259	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pMD2.G
  (Search Vector Database)
• Backbone size w/o insert (bp): 5824
• Vector type: Mammalian Expression, Lentiviral ; Envelope

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSV G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Fwd

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 880 References

Depositor Comments

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please note that the full sequence for this plasmid is approximated and not fully verified (e.g. there is no ClaI site in this plasmid). Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.