PurposeVSV-G envelope expressing plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12259||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Vector typeMammalian Expression, Lentiviral ; Envelope
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameVSV G
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Fwd (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this plasmid runs as a dimer (>11kb). While this may reduce DNA yield, the plasmid still functions as expected for viral packaging.
Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259 ; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)