pMD2.G
(Plasmid #12259)

• Purpose: VSV-G envelope expressing plasmid
• Depositing Lab: Didier Trono
• Publication: Trono Lab Packaging and Envelope Plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	12259	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pMD2.G
  (Search Vector Database)
• Backbone size w/o insert (bp): 5824
• Vector type: Mammalian Expression, Lentiviral ; Envelope

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSV G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Fwd

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest- NotI, HindIII
  • Addgene Diagnostic Digest- BamHI
  • Addgene Diagnostic Digest- PvuI-HF and ApaI
  • Addgene Diagnostic Digest- EcoRI
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 1752 References

Depositor Comments

Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.