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(Plasmid #12253)


Item Catalog # Description Quantity Price (USD)
Plasmid 12253 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4174
  • Vector type
    Mammalian Expression, Lentiviral ; Packaging

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Entrez Gene
    rev (a.k.a. HIV1gp6)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.

Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.

Please visit the Trono lab for cloning strategies, protocols, publications, and more. See LentiWeb for discussions on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSV-Rev was a gift from Didier Trono (Addgene plasmid # 12253 ; ; RRID:Addgene_12253)
  • For your References section:

    A third-generation lentivirus vector with a conditional packaging system. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov . 72(11):8463-71. PubMed 9765382