Purpose(Empty Backbone) 2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10703
Vector typeMammalian Expression, Lentiviral ; Packaging
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.
Note: This plasmid may run as a dimer. Try testing multiple colonies to select the monomer. It may be helpful to follow protocols for low copy plasmids.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260)