U6 pShuttle
(Plasmid #13428)

• Purpose: (Empty Backbone)
• Depositing Lab: Ronald Kahn
• Publication: pShuttle vectors (unpublished)

Backbone

• Vector backbone: pShuttle
• Modifications to backbone: human U6 promoter inserted into pShuttle
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LKO.1 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The U6 promoter was cloned from Imgenex's plasmid.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression.