Purpose(Empty Backbone) Adenoviral vector for use in AdEasy System.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16400||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepAdEasy and reg-1
- Backbone size (bp) 33414
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-1.
Plasmids recombined in pAdEasy-1 should be packaged in either 293 or 911 cells to make virus.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
AdEasy is a registered trademark of the Johns Hopkins University.
NOTE: Addgene was unable to obtain high quality sequence for this plasmid, which may due to its size and repeat regions. Please take this into consideration when conducting your own quality control.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAdEasy-1 was a gift from Bert Vogelstein (Addgene plasmid # 16400 ; http://n2t.net/addgene:16400 ; RRID:Addgene_16400)
For your References section:A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916