Purpose(Empty Backbone) Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16402||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6736
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pShuttle was a gift from Bert Vogelstein (Addgene plasmid # 16402 ; http://n2t.net/addgene:16402 ; RRID:Addgene_16402)
For your References section:A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916