|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13519||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1235
Entrez GeneMAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Please note there are 3 additional mutations found in the QC sequence that are not present in the NGS full sequencing result. These mutations have no affect on the plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3-Flag MKK6(K82A) was a gift from Roger Davis (Addgene plasmid # 13519 ; http://n2t.net/addgene:13519 ; RRID:Addgene_13519)
For your References section:MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Raingeaud J, Whitmarsh AJ, Barrett T, Derijard B, Davis RJ. Mol Cell Biol. 1996 Mar . 16(3):1247-55. PubMed 8622669