pMG
(Plasmid #138264)

• Purpose: Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
• Depositing Lab: Xiao Wang
• Publication: Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013.

Backbone

• Vector backbone: pUC19
• Vector type: Yeast Expression, CRISPR
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: eGFP flanked by iCas9 sites
• Species: Synthetic
• Promoter: Tef1

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: mCherry
• Promoter: Tef1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry