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RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration.
Standage-Beier K, Brookhouser N, Balachandran P, Zhang Q, Brafman DA, Wang X
CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138257pRS424-ysg(G:H)Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system
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138258pRS424-ysg(NT)Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system
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138259pUC19-hsg(G:H)Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
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138260pUC19-hsg(NT:NT)Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system
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138261pSB1C3-FeGFPDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
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138262pSB1C3-hsg(M)Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system
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138263pSB1C3-ysg(NT)Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.
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138264pMGYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
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138265pUC19-EAMPmCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.
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138266p415:iCas9Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.
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138267pEF:iCas9Expression of mTN3-GGS6-dCas9 (iCas9) in human.
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