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Addgene

pEF:iCas9
(Plasmid #138267)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138267 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTN3-GGS6-dCas9
  • Alt name
    iCas9
  • Species
    Synthetic
  • Mutation
    Cas9-D10A, H840A
  • Promoter Ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF:iCas9 was a gift from Xiao Wang (Addgene plasmid # 138267 ; http://n2t.net/addgene:138267 ; RRID:Addgene_138267)

  • For your References section:

    RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration. Standage-Beier K, Brookhouser N, Balachandran P, Zhang Q, Brafman DA, Wang X. CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013. 10.1089/crispr.2019.0013 PubMed 31436506
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