pT2Apuro-TRE-synPCB2.0
(Plasmid
#139481)
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PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
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Depositing Lab
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Depositing OrganizationNational Institute for Basic Biology
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 139481-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 139481-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepT2Apuro-TRE
- Backbone size w/o insert (bp) 5208
- Total vector size (bp) 8379
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Vector typeMammalian Expression ; Tol2 transposon donor vector
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
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Alt namesynPCB2.0
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SpeciesSynthetic; Thermosynechococcus elongatus bp-1
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Insert Size (bp)3171
- Promoter Tet responsive element
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer not determined
- 3′ sequencing primer GGGCCCTCACATTGCCAAA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 139481-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 139481-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2Apuro-TRE-synPCB2.0 was a gift from Kazuhiro Aoki (Addgene plasmid # 139481 ; http://n2t.net/addgene:139481 ; RRID:Addgene_139481) -
For your References section:
Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K. ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9. 10.1021/acschembio.0c00477 PubMed 33164485
