7iBi89 (rat fibronectin)
(Plasmid
#14065)
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in the United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 14065-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 14065-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBAGH.Sv
- Backbone size w/o insert (bp) 7700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRat Fibronectin 7iBi89
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Alt nameFibronectin 1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3115
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MutationInsert was cut out of pGEM3 with SalI and SacI and blunt end cloned into BamHI sites.
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GenBank IDL20801
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Entrez GeneFn1 (a.k.a. FIBNEC, fn-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer na
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 14065-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 14065-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
7iBi89 (rat fibronectin) was a gift from Richard Hynes (Addgene plasmid # 14065 ; http://n2t.net/addgene:14065 ; RRID:Addgene_14065) -
For your References section:
Elements regulating an alternatively spliced exon of the rat fibronectin gene. Huh GS, Hynes RO. Mol Cell Biol. 1993 Sep . 13(9):5301-14. PubMed 8355683
Map uploaded by the depositor.
