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(Plasmid #14906)


Item Catalog # Description Quantity Price (USD)
Plasmid 14906 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    HLA-A (a.k.a. HLAA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer SV40pro-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A2 from Harry Orr; K^b from Bruce Wallace
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A Hind III-Bgl II fragment of the genomic clone of HLA-A2.1, which contained the genetic information coding for the leader sequence, alphal and alpha2 domains of the HLA-A2.1 molecule, was ligated to a Bam HI-Bam HI fragment excised from the Kb gene, which contained the alpha3, cytoplasmic, and transmembrane regions of the Kb molecule . The A2/Kb construct was purified and ligated into the Bam HI-Hind III sites of pSV2. The depositor notes that this contains the HLA-A2.1 Promoter, and was used for making the transgenic mouse in Vitiello A

This construct was originally described as conferring Neomycin resistance, but based on user feedback, we are no longer confident that the Neo-resistance gene is intact.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HLA-A2/K^b was a gift from Linda Sherman (Addgene plasmid # 14906 ; ; RRID:Addgene_14906)
  • For your References section:

    Species-restricted interactions between CD8 and the alpha 3 domain of class I influence the magnitude of the xenogeneic response. Irwin MJ, Heath WR, Sherman LA. J Exp Med. 1989 Oct 1. 170(4):1091-101. 10.1084/jem.170.4.1091 PubMed 2477484