HLA-A2/K^b
(Plasmid #14906)

• Depositing Lab: Linda Sherman
• Depositing Organization: Scripps California (The Scripps Research Institute)
  Located in United States of America
• Publication: Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.
• Citations: 2 Articles

Backbone

• Vector backbone: pSV2neo
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HLA-A2/K^b
• Alt name: HLA-A2.1
• Alt name: H-2K^b
• Alt name: HLA-A
• Species: H. sapiens (human)
• Insert Size (bp): 8200
• Entrez Gene: HLA-A (a.k.a. HLAA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: SV40pro-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A2 from Harry Orr; K^b from Bruce Wallace

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A Hind III-Bgl II fragment of the genomic clone of HLA-A2.1, which contained the genetic information coding for the leader sequence, alphal and alpha2 domains of the HLA-A2.1 molecule, was ligated to a Bam HI-Bam HI fragment excised from the Kb gene, which contained the alpha3, cytoplasmic, and transmembrane regions of the Kb molecule . The A2/Kb construct was purified and ligated into the Bam HI-Hind III sites of pSV2. The depositor notes that this contains the HLA-A2.1 Promoter, and was used for making the transgenic mouse in Vitiello A et.al.

This construct was originally described as conferring Neomycin resistance, but based on user feedback, we are no longer confident that the Neo-resistance gene is intact.

DNA (Catalog # 14906-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 14906-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.