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pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS
(Plasmid #158741)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158741 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 158741-D50 50 μg of DNA in Tris buffer $495
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DNA 158741-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCD5
  • Backbone manufacturer
    -
  • Total vector size (bp) 5495
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hemagglutinin A/MI/45/15 H1N1
  • Mutation
    AA61-469
  • GenBank ID
    MK622940
  • Promoter CMV
  • Tag / Fusion Protein
    • GCN4-TEV-sfGFP-TwinStrep (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG C
  • 3′ sequencing primer TTC AGG GGG AGG TGT GGG AG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid may run as a dimer. Please try testing multiple colonies to isolate the monomer.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS was a gift from Robert de Vries (Addgene plasmid # 158741 ; http://n2t.net/addgene:158741 ; RRID:Addgene_158741)

  • For your References section:

    Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells. van der Woude R, Turner HL, Tomris I, Bouwman KM, Ward AB, de Vries RP. Protein Sci. 2020 Jul 25. doi: 10.1002/pro.3918. 10.1002/pro.3918 PubMed 32710576
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