pCD5-PR8-TEV-sfGFP-twinStrep
(Plasmid #157933)

• Purpose: Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP
• Depositing Lab: Robert de Vries
• Publication: Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856. doi: 10.1016/j.jmb.2018.12.014. Epub 2018 Dec 29.

Backbone

• Vector backbone: pCD5
• Backbone manufacturer: -
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 6500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: A/PuertoRico/8/34 hemagglutinin
• Species: Synthetic
• Insert Size (bp): 1500
• Mutation: E190D
• Entrez Gene: HA (a.k.a. FLUAVs4gp1)
• Promoter: CMV
• Tag / Fusion Protein:
  • sfGFP & TwinStrep (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: GGC TAA CTA GAG AAC CCA CTG C
• 3′ sequencing primer: TTC AGG GGG AGG TGT GGG AG

Resource Information

• Supplemental Documents:
  • pCD5 vectors in MC1061 bugs

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry