pET28a 6xHis PreScission SARS-CoV-2 nsp13
PurposeExpresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||159390||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5297
- Total vector size (bp) 7108
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Gene/Insert nameN-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
Insert Size (bp)1812
- Promoter T7
/ Fusion Protein
- His6 tag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byGenScript
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Protein sequence was optimized by GenScript for insect cell expression using the OptimumGene Codon Optimization Analysis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a 6xHis PreScission SARS-CoV-2 nsp13 was a gift from Tarun Kapoor (Addgene plasmid # 159390 ; http://n2t.net/addgene:159390 ; RRID:Addgene_159390)
For your References section:Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex. Chen J, Malone B, Llewellyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, Eng ET, Vatandaslar H, Chait B, Kapoor T, Darst SA, Campbell EA. bioRxiv. 2020 Jul 13. doi: 10.1101/2020.07.08.194084. 10.1101/2020.07.08.194084 PubMed 32676607